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Plasma Sci. Technol. ›› 2018, Vol. 20 ›› Issue (11): 115503.doi: 10.1088/2058-6272/aacaee

• Plasma Technology • Previous Articles     Next Articles

Experimental study on bacteria disinfection using a pulsed cold plasma jet with helium/ oxygen mixed gas

Guanlei DENG (邓官垒) 1, Qikang JIN (金杞糠) 1, Shengyong YIN (殷胜勇) 2, Chao ZHENG (郑超) 3, Zhen LIU (刘振) 1 and Keping YAN (闫克平)1   


  1. 1 Institute of Industrial Ecology and Environment, Zhejiang University, Hangzhou 310007, People’s Republic of China
    2 Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, People’s Republic of China
    3 Zhejiang Center for Medical Device Adverse Events Monitoring and Safety Research, Hangzhou 310009, People’s Republic of China
  • Received:2018-04-11 Published:2018-06-06
  • Supported by:

    This work is supported by National Natural Science Foundation of China (No. 51377145) and the Science and Technology Program of Zhejiang Province China (Nos. 2015F10011 and 2014C33022).


Cold atmospheric plasma jet is widely used in many fields due to the reactive oxygen species and low temperature for heat-sensitive products. This paper presents the inactivation of bacteria via a pulsed plasma jet with He/O2 mixed gas. To evaluate the disinfection performance, Staphylococcus aureus was used as an indicator bacteria for experiments. When the plasma jet dealt with agar plates spraying bacteria, it was found that mixed gas has a better performance than pure inert gas, indicated by the disinfection area. The increment of oxygen gas addition was beneficial to the disinfection ability of the plasma jet, while the gas had an opposite effect on the length of jet production. The experiments showed the efficacy of Staphylococcus aureus disinfection could reach up to 99.47% via a helium/oxygen (2%) plasma jet.

Key words: plasma jet, non-thermal plasma, disinfection, ROS