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Plasma Sci. Technol. ›› 2018, Vol. 20 ›› Issue (4): 044001.doi: 10.1088/2058-6272/aa9479

• Plasma Biomedicine • Previous Articles     Next Articles

Measurement of cytoplasmic Ca2+ concentration in Saccharomyces cerevisiae induced by air cold plasma

Xiaoyu DONG (董晓宇)   

  

  1. School of Life Science and Biotechnology, Dalian University, Dalian 116622, People’s Republic of China
  • Received:2017-08-12 Published:2017-10-15
  • Supported by:

    This work was supported by National Natural Science Foundation of China (Grant Nos. 21246012, 21306015 and 21476032).

Abstract:

In this study, a novel approach to measure the absolute cytoplasmic Ca2+ concentration ([Ca2+]cyt) using the Ca2+ indicator fluo-3 AM was established. The parameters associated with the probe fluo-3 AM were optimized to accurately determine fluorescence intensity from the Ca2+-bound probe. Using three optimized parameters (final concentration of 6 mM probe, incubation time of 135 min, loading probe before plasma treatment), the maximum fluorescence intensity (Fmax=527.8 a.u.) and the minimum fluorescence intensity (Fmin=63.8 a.u.) were obtained in a saturated Ca2+ solution or a solution of lacking Ca2+. Correspondingly, the maximum [Ca2+]cyt induced by cold plasma was 1232.5 nM. Therefore, the Ca2+ indicator fluo-3 AM was successfully applied to measure the absolute [Ca2+]cyt in Saccharomyces cerevisiae stimulated by cold plasma at atmospheric air pressure.

Key words: cytoplasmic Ca2+ concentration, Saccharomyces cerevisiae, air cold plasma, parameter optimization