Experimental Research on the Sterilization of Escherichia Coli and Bacillus Subtilis in Drinking Water by Dielectric Barrier Discharge

LI Yang (李洋); YI Chengwu (依成武); LI Jingjing (李京京); YI Rongjie (依蓉婕); WANG Huijuan (王慧娟)

doi:10.1088/1009-0630/18/2/13

Plasma Science and Technology > 2016 > 18(2): 173-178. > DOI: 10.1088/1009-0630/18/2/13

LI Yang (李洋), YI Chengwu (依成武), LI Jingjing (李京京), YI Rongjie (依蓉婕), WANG Huijuan (王慧娟). Experimental Research on the Sterilization of Escherichia Coli and Bacillus Subtilis in Drinking Water by Dielectric Barrier Discharge[J]. Plasma Science and Technology, 2016, 18(2): 173-178. DOI: 10.1088/1009-0630/18/2/13

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Experimental Research on the Sterilization of Escherichia Coli and Bacillus Subtilis in Drinking Water by Dielectric Barrier Discharge

School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China

Funds: supported by the Science and Technology Support Project Plan and Social Development of Jiangsu Province, China (No. BE2011732), the Science and Technology Support Project Plan and Social Development of Zhenjiang, Jiangsu, China(No. SH2012013)

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Received Date: January 18, 2015

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Abstract

Abstract

The bactericidal effect on the representative type of Gram-negative Escherichia coli (E. coli) and Gram-positive Bacillus subtilis in drinking water was investigated in this paper by using dielectric barrier discharge (DBD) advanced oxidation technology. The sterilizing rates under different conditions of reaction time t, input voltage V, pH value, and initial concentration of bacteria C0 were investigated to figure out the optimum sterilization conditions. Our observations and comparisons of cell morphology alteration by scanning electron microscopy and transmission electron microscopy revealed the sterilization mechanisms. The results showed that the sterilizing rate increased obviously with the extension of reaction time t and the rise of input voltage V . The optimal sterilization effect was achieved when the pH value was 7.1. As the initial concentration of bacteria rose, the sterilizing rate decreased. When the input voltage was 2.2 kV and the initial concentration of bacteria was relatively low, the sterilizing rate almost reached 100% after a certain treatment time in neutral aqueous solution. The reasons for the great damage of cell structure and the killing of bacteria are the oxidation of O₃, OH and the accumulation of active species produced by DBD. The article provides a certain theoretical and experimental basis for DBD application in water pollution treatment.
- E. coli,
- BSN,
- drinking water,
- active species,
- sterilizing rate

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References

1 Van Grieken R, Marug′ an J, Pablos C, et al. 2010, Applied Catalysis B: Environmental, 100: 212 2 Cui Yuejuan, Yuan Jian, Yuan Hongying, et al. 2012,Sichuan Environment, 31: 104 (in Chinese) 3 Oh B T, Seo Y S, Sudhakar D, et al. 2014, J. Hazard.Mater., 279: 105 4 Chang E E, Liu T Y, Huang C P, et al. 2012, Separation and Purification Technology, 98: 123 5 Liu Huanhuan, Chen Quanyuan, Yu Yang, et al. 2013,J. Hazard. Mater., 263: 593 6 Ndong L B B, Gu X G, Lu S G, et al. 2015, Chemical Engineering Science, 123: 367 7 Zhang Hong, Yang Linfang, Yu Zengliang, et al. 2014,J. Hazard. Mater., 268: 33 8 Wang Huijuan, Li Jie, Quan Xie, et al. 2007, J. Hazard. Mater., 141: 336 9 Jayasena D D, Kim H J, Yong H I, et al. 2015, Food Microbiol., 46: 51 10 Moreira A J, Mansano R D, PintoT J A, et al. 2004,Applied Surface Science, 235: 151 11 Shinde S S, Bhosale C H, Rajpure K Y. 2012, Journal of Photochemistry and Photobiology B: Biology, 116:66 12 Cheon H L, Shin J Y, Park K H, et al. 2015, Food Control, 50: 441 13 Jiang Bo, Zheng Jingtang, Qiu Shi, et al. 2014, Chemical Engineering Journal, 236: 348 14 Fresno L, Fondevila D, Bambo O, et al. 2010, Veterinary Journal, 185: 322 15 Deng Sanxi, Cheng Cheng, Ni Guohua, et al. 2010,Current Applied Physics, 10: 1164 16 Hu Miao, Guo Yun. 2011, Applied Surface Science,257: 7065 17 Zhao Y, Singh M K, Ogino A, et al. 2010, Thin Solid Films, 518: 3590 18 Yi Chengwu, Li Qianqian, He Cong, et al. 2011, Journal of Ecology and Rural Environment, 27: 65 (in Chinese) 19 Wang Xiaoyan, Zhou Minghua, Jin Xinglong. 2012,Electrochimica Acta, 83: 501 20 Sureshkumar A, Sankar R, Mandal M, et al. 2010, International Journal of Pharmaceutics, 396: 17 21 S′ anchez-Polo M, Von Gunten U, Rivera-Utrilla J.2005, Water Research, 39: 3189 22 Foster H A, Ditta I B, Varghese S, et al. 2011, Applied Microbiology and Biotechnology, 90: 1847 23 Gogniat G, Dukan S. 2007, Appl. Environ. Microbiol., 73: 7740 24 Marsili L, Espie S, Anderson J G, et al. 2002, Radiation Physics and Chemistry, 65: 507 25 Zhang Zhitao, Bai Mindong, Bai Xiyao, et al. 2004,Journal of Advanced Oxidation Technologies, 7: 178 26 Xu Wei, Yi Rongjie, Li Jingjing, et al. 2014, Advanced Materials Research, 864: 418 27 Linley E, Denyer S P, McDonnell G, et al. 2012, Journal of Antimicrobial Chemotherapy, 67: 1589 28 Guo Jian, Huang Kang, Wang Jianping. 2015, Food Control, 50: 482

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