Cold atmospheric-pressure air plasma treatment of C6 glioma cells: effects of reactive oxygen species in the medium produced by the plasma on cell death

Yuyang WANG (汪宇扬); Cheng CHENG (程诚); Peng GAO (高鹏); Shaopeng LI (李少鹏); Jie SHEN (沈洁); Yan LAN (兰彦); Yongqiang YU (余永强); Paul K CHU (朱剑豪)

doi:10.1088/2058-6272/19/2/025503

Plasma Science and Technology > 2017 > 19(2): 25503-025503. > DOI: 10.1088/2058-6272/19/2/025503

Yuyang WANG (汪宇扬), Cheng CHENG (程诚), Peng GAO (高鹏), Shaopeng LI (李少鹏), Jie SHEN (沈洁), Yan LAN (兰彦), Yongqiang YU (余永强), Paul K CHU (朱剑豪). Cold atmospheric-pressure air plasma treatment of C6 glioma cells: effects of reactive oxygen species in the medium produced by the plasma on cell death[J]. Plasma Science and Technology, 2017, 19(2): 25503-025503. DOI: 10.1088/2058-6272/19/2/025503

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Cold atmospheric-pressure air plasma treatment of C6 glioma cells: effects of reactive oxygen species in the medium produced by the plasma on cell death

1 The first Affiliated Hospital of Anhui Medical University, Hefei 230032, People’s Republic of China 2 Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, People’s Republic of China 3 Department of Physics and Materials Science, City University of Hong Kong, TatChee Avenue, Kowloon, Hong Kong, People’s Republic of China

Funds: This work was jointly supported by the Education and Research Foundation of Anhui Province (KJ2015A327), Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences No. DSJJ-14-YY02, as well as Hong Kong Research Grants Council (RGC) General Research Funds (GRF) No. CityU 11301215.

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Received Date: August 27, 2016

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Abstract

Abstract

An atmospheric-pressure air plasma is employed to treat C6 glioma cells in vitro. To elucidate on the mechanism causing cell death and role of reactive species (RS) in the medium produced by the plasma, the concentration of the long-lived RS such as hydrogen peroxide, nitrate, and ozone in the plasma-treated liquid (phosphate-buffered saline solution) is measured. When vitamin C is added to the medium as a ROS quencher, the viability of C6 glioma cells after the plasma treatment is different from that without vitamin C. The results demonstrate that reactive oxygen species (ROS) such as H₂O₂, and O₃ constitute the main factors for inactivation of C6 glioma cells and the reactive nitrogen species (RNS) may only play an auxiliary role in cell death.
- atmospheric pressure air plasma,
- reactive species,
- cell death

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References (32)

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