1 Institute of Public Health, Ljubljanska bb, 81000 Podgorica, Montenegro
2 Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia, EU
3 Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
4 Institute of Nanoscience and Nanotechnology (INN), National Center for Scientific Research NCSR Demokritos, Aghia Paraskevi, Attiki, Greece
Funds: The financial support from the Slovenian Research Agency (ARRS), NATO CLG/SPS.984555 and EU COST grant MP1101 is gratefully acknowledged. NP, SL and ZLP are grateful to the MESS 171037 and 41011 projects for partial support..
The degradation of Escherichia coli bacteria by treatment with cold, weakly ionised, highly dissociated oxygen plasma, with an electron temperature of 3 eV, a plasma density of 8×1015 m−3 and a neutral oxygen atom density of 3.5×1021 m−3 was studied. To determine the ‘real’ plasma effects, two methods were used for evaluation and determination, as well as a comparison of the number of bacteria that had survived: the standard plate count technique (PCT) and advanced fluorescence-activated cell sorting (FACS). Bacteria were deposited onto glass substrates and kept below 50 °C during the experiments with oxygen plasma. The results showed that the bacteria had fully degraded after about 2 min of plasma treatment, depending slightly on the amount of bacteria that had been?deposited on the substrates. The very precise determination of the O flux on the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall—about 6×1024 m−2—as well as deactivation of the substrates—about 8×1025 m−2. These results were taken in order to discuss other results obtained by comparable studies and scientific method?evaluations in the determination of plasma effects on bacteria.
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