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Danijela VUJOŠEVIC, Uroš CVELBAR, Urška REPNIK, Martina MODIC, Saša LAZOVIC, Tina ZAVAŠNIK-BERGANT, Nevena PUAC, Boban MUGOŠA, Evangelos GOGOLIDES, Zoran Lj PETROVIC, Miran MOZETIC. Plasma effects on the bacteria Escherichia coli via two evaluation methods[J]. Plasma Science and Technology, 2017, 19(7): 75504-075504. DOI: 10.1088/2058-6272/aa656b
Citation: Danijela VUJOŠEVIC, Uroš CVELBAR, Urška REPNIK, Martina MODIC, Saša LAZOVIC, Tina ZAVAŠNIK-BERGANT, Nevena PUAC, Boban MUGOŠA, Evangelos GOGOLIDES, Zoran Lj PETROVIC, Miran MOZETIC. Plasma effects on the bacteria Escherichia coli via two evaluation methods[J]. Plasma Science and Technology, 2017, 19(7): 75504-075504. DOI: 10.1088/2058-6272/aa656b

Plasma effects on the bacteria Escherichia coli via two evaluation methods

Funds: The financial support from the Slovenian Research Agency (ARRS), NATO CLG/SPS.984555 and EU COST grant MP1101 is gratefully acknowledged. NP, SL and ZLP are grateful to the MESS 171037 and 41011 projects for partial support..
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  • The degradation of Escherichia coli bacteria by treatment with cold, weakly ionised, highly dissociated oxygen plasma, with an electron temperature of 3 eV, a plasma density of 8×1015 m−3 and a neutral oxygen atom density of 3.5×1021 m−3 was studied. To determine the ‘real’ plasma effects, two methods were used for evaluation and determination, as well as a comparison of the number of bacteria that had survived: the standard plate count technique (PCT) and advanced fluorescence-activated cell sorting (FACS). Bacteria were deposited onto glass substrates and kept below 50 °C during the experiments with oxygen plasma. The results showed that the bacteria had fully degraded after about 2 min of plasma treatment, depending slightly on the amount of bacteria that had been?deposited on the substrates. The very precise determination of the O flux on the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall—about 6×1024 m−2—as well as deactivation of the substrates—about 8×1025 m−2. These results were taken in order to discuss other results obtained by comparable studies and scientific method?evaluations in the determination of plasma effects on bacteria.
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