Efficacy of Atmospheric Pressure Plasma as an Antibacterial Agent Against Enterococcus Faecalis in Vitro

CAO Yingguang; YANG Ping; LU Xinpei; XIONG Zilan; YE Tao; XIONG Qing; SUN Ziyong

Plasma Science and Technology > 2011 > 13(1): 93-98.

CAO Yingguang, YANG Ping, LU Xinpei, XIONG Zilan, YE Tao, XIONG Qing, SUN Ziyong. Efficacy of Atmospheric Pressure Plasma as an Antibacterial Agent Against Enterococcus Faecalis in Vitro[J]. Plasma Science and Technology, 2011, 13(1): 93-98.

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Efficacy of Atmospheric Pressure Plasma as an Antibacterial Agent Against Enterococcus Faecalis in Vitro

1 Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2 College of Electrical and Electronics Engineering, Huazhong University of Science and Technology, Wuhan 430074, China 3 Department of Clinical Laboratory, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Funds: Supported by the National Natural Science Foundation of China (No. 10875048).

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Corresponding author:
LU Xinpei (卢新培), Email: luxinpei@hotmail.com, Tel: 86-027-87541346.

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Abstract

Abstract

Enterococcus faecalis (E. faecalis) is a microorganism that can survive extreme challenges in obturated root canals. The aim of this study was to evaluate the efficacy of a non-thermal atmospheric pressure plasma plume against E. faecalis in vitro. A non-thermal atmospheric pressure plasma jet device which could generate a cold plasma plume carrying a peak current of 300 mA was used. The antibacterial efficacy of this device against E. faecalis and its biofilm under different conditions was detected. The antibacterial efficacy of the plasma against E. faecalis and Staphylococcus aureus (S. aureus) was also evaluated. After plasma treatment, the average diameter of inhibition zone on S. aureus and E. faecalis was 2.62±0.26 and 1.06±0.30 cm, respectively (P < 0.05). The diameter was increased with prolongation of the treatment duration. The diameters of inhibition zone of the sealed Petri dishes were larger than those of the uncovered Petri dishes. There was significant difference in colony-forming units between plasma group and control group on E. faecalis biofilm (P < 0.01). The transmission electron microscopy revealed that the ultrastructural changes cytoderm of E. faecalis were observed after treatment for 2 min. It is concluded that the non-thermal atmospheric pressure plasma could serve as an effective adjunct to standard endodontic microbial treatment.
- non-thermal atmospheric pressure plasma jet,
- Enterococcus faecalis,
- Staphylococcus aureus

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