Abstract: In this study, the effects of plasma treatment parameters on surface morphology, chemical constituent, dyeability and color fastness of silk fabric were investigated. Atmospheric pressure glow discharge plasma generated with different applied voltages (0 kV to 45 kV) was used to treat the surface of silk fabrics. C I Natural Yellow 3 was used to dye untreated and plasmatreated silk fabrics. The physical analysis based on scanning electron microscopy showed that the surface of silk fabrics was affected by plasma treatment. The chemical analysis was investigated with x-ray photoelectron spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy. The results showed that the content of C 1s decreased with the increasing applied voltage, the content of N 1s and O 1s increased with the increasing applied voltage. The increasing K/S values represented that the dyeability of silk fabrics was improved after plasma treatment. The color fastness to dry and wet rubbing was decreased after plasma treatment.