Study of Bacterial Samples Using Laser Induced Breakdown Spectroscopy

W A FAROOQ; M ATIF; W TAWFIK; M S ALSALHI; Z A ALAHMED; M SARFRAZ; J P SINGH

doi:10.1088/1009-0630/16/12/10

Plasma Science and Technology > 2014 > 16(12): 1141-1146. > DOI: 10.1088/1009-0630/16/12/10

W A FAROOQ, M ATIF, W TAWFIK, M S ALSALHI, Z A ALAHMED, M SARFRAZ, J P SINGH. Study of Bacterial Samples Using Laser Induced Breakdown Spectroscopy[J]. Plasma Science and Technology, 2014, 16(12): 1141-1146. DOI: 10.1088/1009-0630/16/12/10

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Study of Bacterial Samples Using Laser Induced Breakdown Spectroscopy

1Physics and Astronomy Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia 2National Institute of Laser and Optronics, Nilore, Islamabad, Pakistan 3National Institute of Laser Enhanced Sciences NILES, Department of Environmental Applications, Cairo University, Cairo, Egypt 4Sustainable Energy Technologies Centre, King Saud University, Riyadh, Saudi Arabia 5Institute for Clean Energy Technology, Mississippi State University, Starkville, MS 39759, USA

Funds: supported by King Saud University, Deanship of Scienti¯c Research, College of Science Research Center

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Received Date: December 26, 2013

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Abstract

Laser-induced breakdown spectroscopy (LIBS) technique has been applied to inves- tigate two different types of bacteria, Escherichia coli (B1) and Micrococcus luteus (B2) deposited on glass slides using Spectrolaser 7000. LIBS spectra were analyzed using spectrolaser software. LIBS spectrum of glass substrate was compared with bacteria spectra. Ca, Mg, Na, K, P, S, Cl, Fe, Al, Mn, Cu, C, H and CN-band appeared in bacterial samples in air. Two carbon lines at 193.02 nm, 247.88 nm and one hydrogen line at 656.28 nm with intensity ratios of 1.9, 1.83 and 1.53 appeared in bacterial samples B1 and B2 respectively. Carbon and hydrogen are the impor- tant components of the bio-samples like bacteria and other cancer cells. Investigation on LIBS spectra of the samples in He and Ar atmospheres is also presented. Ni lines appeared only in B2 sample in Ar atmosphere. From the present experimental results we are able to show that LIBS technique has a potential in the identification and discrimination of different types of bacteria.
- laser induced breakdown,
- bacteria,
- bio-samples and carbon lines

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