Effect of Cold Plasma on Cell Viability and Collagen Synthesis in Cultured Murine Fibroblasts

SHI Xingmin (石兴民); CAI Jingfen (蔡晶芬); XU Guimin (许桂敏); REN Hongbin (任鸿斌); CHEN Sile (陈思乐); CHANG Zhengshi (常正实); LIU Jinren (刘进仁); HUANG Chongya (黄崇亚); ZHANG Guanjun (张冠军); WU Xili (吴喜利)

doi:10.1088/1009-0630/18/4/04

Plasma Science and Technology > 2016 > 18(4): 353-359. > DOI: 10.1088/1009-0630/18/4/04

SHI Xingmin (石兴民), CAI Jingfen (蔡晶芬), XU Guimin (许桂敏), REN Hongbin (任鸿斌), CHEN Sile (陈思乐), CHANG Zhengshi (常正实), LIU Jinren (刘进仁), HUANG Chongya (黄崇亚), ZHANG Guanjun (张冠军), WU Xili (吴喜利). Effect of Cold Plasma on Cell Viability and Collagen Synthesis in Cultured Murine Fibroblasts[J]. Plasma Science and Technology, 2016, 18(4): 353-359. DOI: 10.1088/1009-0630/18/4/04

Citation:

PDF (314 KB)

Effect of Cold Plasma on Cell Viability and Collagen Synthesis in Cultured Murine Fibroblasts

1School of Public Health, Medical Science Center, Xi’an Jiaotong University, Xi’an 710061, China 2State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi’an Jiaotong University, Xi’an 710049, China 3Second A?liated Hospital, Xi’an Jiaotong University, Xi’an 710004, China

Funds: supported partly by National Natural Science Foundation of China (Nos.81372076, 51307133 and 51221005), China National Funds for Distinguished Young Scientists (No. 51125029), the Sci-Tech Project of Shaanxi Province of China (No. 2010K16-04), and the Fundamental Research Funds for the Central Universities of China (No. xkjc2013004)

More Information

Received Date: August 31, 2015

Graphical Abstract

Abstract

Abstract

An argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro. Experimental results showed that, compared with the control cells, the treatment of fibroblasts with 15 s of plasma led to a significant increase of cell viability and collagen synthesis, while the treatment of 25 s plasma resulted in a remarkable decrease. Exploration of related mechanisms suggested that cold plasma could up-regulate CyclinD1 gene expression and down-regulate p27 gene expression at a low dose, while it could down-regulate CyclinD1 expression and up-regulate p27 expression at a higher dose, thus altering the cell cycle progression, and then affecting cell viability and collagen synthesis of fibroblasts.
- cold plasma,
- cell viability,
- collagen synthesis,
- fibroblasts

FullText(HTML)

References (1)

References

1 Tipa R S, Kroesen G M W. 2011, IEEE Trans. Plasma Sci., 39: 2978 2 Orazov M, Sakiyama Y, Graves D B. 2012, J. Phys.D: Appl. Phys., 45: 445201 3 Nastuta A V, Topala I, Grigoras C, et al. 2011, J.Phys. D: Appl. Phys., 44: 105204 4 Salehi S, Shokri A, Khani M R, et al. 2015, Biointerphases, 10: 029504 5 Isbary G, Heinlin J, Shimizu T, et al. 2012, Br. J. Dermatol., 167: 404 6 Chernets N, Zhang J, Steinbeck M J, et al. 2015, Tissue Eng.: Part A, 21: 300 7 Thi M-H N, Shao P-L, Liao J-D, et al. 2014, Plasma Process. Polym., 11: 1076 8 Isbary G, Morfill G, Schmidt H U, et al. 2010, Br. J.Dermatol., 163: 78 9 Yu Y, Tan M, Chen H X, et al. 2011, J. Huazhong Univ. Sci. Technol. [Med. Sci.], 31: 390 10 Barton A, Wende K, Bundscherer L, et al. 2013,Plasma Med., 3: 125 11 Bekeschus S, Schmidt A, Bethge L, et al. 2015, Oxidative Med. Cell. Longev., 7: 607969 12 Thackham J A, McElwain D L S, Long R J. 2008,Wound Repair Regen., 16: 321 13 Shi Y, Li D R, Yan G F, et al. 2008, Chongqing Med.,37: 1721 (in Chinese) 14 Kogelschatz U. 2003, IEEE Trans. Plasma Sci., 23: 1 15 Shi X M, Chang Z S, Wu X L, et al. 2013, Plasma Process. Polym., 10: 808 16 Kalghatgi S U, Friedman G, Fridman A, et al. 2010,Ann. Biomed. Eng., 38: 78 17 Izutani Y, Yogosawa S, Sowa Y, et al. 2012, Int. J.Oncol., 40: 816 18 Hensel K, Kucerova K, Tarabova B, et al. 2015, Biointerphases, 10: 029515 19 Volotskova O, Hawley T S, Stepp M A, et al. 2012,Sci. Rep., 2: 636 20 Zhao S, Xiong Z, Mao X, et al. 2012, IEEE Trans.Plasma Sci., 40: 2179 21 Song J D. 2004, Medical Cellular Molecular Biology.China People’s Medical Publishing House, Beijing,p.389 (in Chinese) 22 Graves D B. 2014, Phys. Plasmas, 21: 080901 23 Sensenig R, Kalghatgi S, Cerchar E, et al. 2011, Ann.Biomed. Eng., 39: 674 24 Han G, Nguyen LN, Macherla C, et al. 2012, Am. J.Pathol., 180: 1465 25 Kuo S P, Chen C Y, Lin C S, et al. 2010, IEEE Trans.Plasma Sci., 38: 1908 26 Xu D H, Liu D X, Wang B Q, et al. 2015, PLoS One,10: e0128205 27 Lazovic S, Puac N, Miletic M, et al. 2010, New J.Phys., 12: 083037 28 Shen J, Sun Q, Zhang Z L, et al. 2015, Plasma Process.Polym., 12: 252 29 Zhang H, Xu Z M, Shen J, et al. 2015, Sci. Rep., 5:10031 30 Xu Z M, Wei J, Shen J, et al. 2015, Appl. Phys. Lett.106: 213701 31 Haertel B, Woedtke T, Weltmann K D, et al. 2014, Biomol. Ther., 22: 477

[1]	Pan LU, Dong-Wook KIM, Dong-Wha PARK. Simple reactor for the synthesis of silver nanoparticles with the assistance of ethanol by gas–liquid discharge plasma[J]. Plasma Science and Technology, 2019, 21(4): 44005-044005. DOI: 10.1088/2058-6272/aaeada
[2]	Yuchuan QIN (秦豫川), Shulou QIAN (钱树楼), Cheng WANG (王城), Weidong XIA (夏维东). Effects of nitrogen on ozone synthesis in packed-bed dielectric barrier discharge[J]. Plasma Science and Technology, 2018, 20(9): 95501-095501. DOI: 10.1088/2058-6272/aac203
[3]	Yuyang WANG (汪宇扬), Cheng CHENG (程诚), Peng GAO (高鹏), Shaopeng LI (李少鹏), Jie SHEN (沈洁), Yan LAN (兰彦), Yongqiang YU (余永强), Paul K CHU (朱剑豪). Cold atmospheric-pressure air plasma treatment of C6 glioma cells: effects of reactive oxygen species in the medium produced by the plasma on cell death[J]. Plasma Science and Technology, 2017, 19(2): 25503-025503. DOI: 10.1088/2058-6272/19/2/025503
[4]	Xiaoyu DONG (董晓宇), Tingting LIU (刘婷婷), Yuqin XIONG (熊玉琴). A novel approach to regulate cell membrane permeability for ATP and NADH formation in Saccharomyces cerevisiae induced by air cold plasma[J]. Plasma Science and Technology, 2017, 19(2): 24001-024001. DOI: 10.1088/2058-6272/19/2/024001
[5]	ZHAN Zhibin (詹志彬), DI Lanbo (底兰波), ZHANG Xiuling (张秀玲), LI Yanchun (李燕春). Synthesis of Cu-Doped Mixed-Phase TiO₂ with the Assistance of Ionic Liquid by Atmospheric-Pressure Cold Plasma[J]. Plasma Science and Technology, 2016, 18(5): 494-499. DOI: 10.1088/1009-0630/18/5/09
[6]	Djamel Eddine GOURARI, Manitra RAZAFINIMANANA, Marc MONTHIOUX, Raul ARENAL, Flavien VALENSI, S′ebastien JOULIE, Virginie SERIN. Synthesis of (B-C-N) Nanomaterials by Arc Discharge Using Heterogeneous Anodes[J]. Plasma Science and Technology, 2016, 18(5): 465-468. DOI: 10.1088/1009-0630/18/5/03
[7]	Nasrin NAVABSAFA, Hamid GHOMI, Maryam NIKKHAH, Soheila MOHADES, et al.. Effect of BCD Plasma on a Bacteria Cell Membrane[J]. Plasma Science and Technology, 2013, 15(7): 685-689. DOI: 10.1088/1009-0630/15/7/15
[8]	I. M. ULANOV, M. V. ISUPOV, A. Yu LITVINSEV, P. A. MISCHENKO. Plasma-Chemical Synthesis of Oxide Powders Using Transformer-Coupled Discharge[J]. Plasma Science and Technology, 2013, 15(4): 386-390. DOI: 10.1088/1009-0630/15/4/14
[9]	BAI Suli(白素丽), HUANG Chengdu(黄承都), LV Jing(吕静), LI Zhenhua (李振花). Performance of Cobalt-based Fischer-Tropsch Synthesis Catalysts using Dielectric-Barrier Discharge Plasma as an Alternative to Thermal Calcination?[J]. Plasma Science and Technology, 2012, 14(1): 54-57. DOI: 10.1088/1009-0630/14/1/12
[10]	HUANG Zhijun(黄志军), WU Qingyou (吴青友), LI Xiang (李祥), SHANG Shuyong (尚书勇), DAI Xiaoyan (戴晓雁), YIN Yongxiang (印永祥). Synthesis and Characterization of Nano-sized Boron Powder Prepared by Plasma Torch[J]. Plasma Science and Technology, 2010, 12(5): 577-580.