Measurement of cytoplasmic Ca<sup>2+</sup> concentration in Saccharomyces cerevisiae induced by air cold plasma

Xiaoyu DONG (董晓宇)

doi:10.1088/2058-6272/aa9479

Plasma Science and Technology > 2018 > 20(4): 44001-044001. > DOI: 10.1088/2058-6272/aa9479

Xiaoyu DONG (董晓宇). Measurement of cytoplasmic Ca²⁺ concentration in Saccharomyces cerevisiae induced by air cold plasma[J]. Plasma Science and Technology, 2018, 20(4): 44001-044001. DOI: 10.1088/2058-6272/aa9479

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Measurement of cytoplasmic Ca²⁺ concentration in Saccharomyces cerevisiae induced by air cold plasma

Xiaoyu DONG (董晓宇)

School of Life Science and Biotechnology, Dalian University, Dalian 116622, People’s Republic of China

Funds: This work was supported by National Natural Science Foundation of China (Grant Nos. 21246012, 21306015 and 21476032).

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Received Date: August 11, 2017

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Abstract

Abstract

In this study, a novel approach to measure the absolute cytoplasmic Ca²⁺ concentration ([Ca²⁺]cyt) using the Ca²⁺ indicator fluo-3 AM was established. The parameters associated with the probe fluo-3 AM were optimized to accurately determine fluorescence intensity from the Ca²⁺-bound probe. Using three optimized parameters (final concentration of 6 mM probe, incubation time of 135 min, loading probe before plasma treatment), the maximum fluorescence intensity (F_max=527.8 a.u.) and the minimum fluorescence intensity (F_min=63.8 a.u.) were obtained in a saturated Ca²⁺ solution or a solution of lacking Ca²⁺. Correspondingly, the maximum
[Ca²⁺]cyt induced by cold plasma was 1232.5 nM. Therefore, the Ca²⁺ indicator fluo-3 AM was successfully applied to measure the absolute
[Ca²⁺]cyt in Saccharomyces cerevisiae stimulated by cold plasma at atmospheric air pressure.
- cytoplasmic Ca²⁺ concentration,
- Saccharomyces cerevisiae,
- air cold plasma,
- parameter optimization

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Measurement of cytoplasmic Ca²⁺ concentration in Saccharomyces cerevisiae induced by air cold plasma

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