Systemic study on the safety of immuno-deficient nude mice treated by atmospheric plasma-activated water

Dehui XU (许德晖); Qingjie CUI (崔庆杰); Yujing XU (许宇静); Bingchuan WANG (王冰川); Miao TIAN (田苗); Qiaosong LI (李乔松); Zhijie LIU (刘志杰); Dingxin LIU (刘定新); Hailan CHEN (陈海兰); Michael G KONG (孔刚玉)

doi:10.1088/2058-6272/aa9842

Plasma Science and Technology > 2018 > 20(4): 44003-044003. > DOI: 10.1088/2058-6272/aa9842

Dehui XU (许德晖), Qingjie CUI (崔庆杰), Yujing XU (许宇静), Bingchuan WANG (王冰川), Miao TIAN (田苗), Qiaosong LI (李乔松), Zhijie LIU (刘志杰), Dingxin LIU (刘定新), Hailan CHEN (陈海兰), Michael G KONG (孔刚玉). Systemic study on the safety of immuno-deficient nude mice treated by atmospheric plasma-activated water[J]. Plasma Science and Technology, 2018, 20(4): 44003-044003. DOI: 10.1088/2058-6272/aa9842

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Systemic study on the safety of immuno-deficient nude mice treated by atmospheric plasma-activated water

1 State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi’an Jiaotong University, Xi’an 710049, People’s Republic of China 2 The school of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, People’s Republic of China 3 Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, United States of America 4 Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23529, United States of America

Funds: This work is supported by National Natural Science Foundation of China (Grant No. 51307135), China Postdoctoral Science Foundation (Grant No. 2017M610639) and the Fundamental Research Funds for Central Universities.

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Received Date: September 03, 2017

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Abstract

Abstract

Cold atmospheric-pressure plasma is a new technology, widely used in many fields of biomedicine, especially in cancer treatment. Cold plasma can selectively kill a variety of tumor cells, and its biological safety in clinical trials is also very important. In many cases, the patient’s immune level is relatively low, so we first studied the safety assessment of plasma treatment in an immunocompromised animal model. In this study, we examined the safety of immuno-deficient nude mice by oral lavage treatment of plasma-activated water, and studied the growth status, main organs and blood biochemical indexes. Acute toxicity test results showed that the maximum dose of plasma treatment for 15 min had no lethal effect and other acute toxicity. There were no significant changes in body weight and survival status of mice after 2 min and 4 min of plasma-activated water (PAW) treatment for 2 weeks. After treatment, the major organs, including heart, liver, spleen, lung and kidney, were not significantly changed in organ coefficient and tissue structure. Blood biochemical markers showed that blood neutrophils and mononuclear cells were slightly increased, and the others remained unchanged. Liver function, renal function, electrolytes, glucose metabolism and lipid metabolism were not affected by different doses of PAW treatment. The above results indicate that PAWtreatment canbeusedtotreat immuno-deficient nude mice without significant safety problems.
- cold atmospheric plasma,
- plasma-activated water,
- immuno-deficient nude mice,
- safety study,
- biochemical testing

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Systemic study on the safety of immuno-deficient nude mice treated by atmospheric plasma-activated water