Comparative transcriptome analysis of atmospheric pressure cold plasma enhanced early seedling growth in <i>Arabidopsis thaliana</i>

Dongjie CUI (崔东洁); Yue YIN (阴悦); Huandong LI (李洹东); Xiaoxia HU (胡小霞); Jie ZHUANG (庄杰); Ruonan MA (马若男); Zhen JIAO (焦浈)

doi:10.1088/2058-6272/ac0686

Plasma Science and Technology > 2021 > 23(8): 85502-085502. > DOI: 10.1088/2058-6272/ac0686

Dongjie CUI (崔东洁), Yue YIN (阴悦), Huandong LI (李洹东), Xiaoxia HU (胡小霞), Jie ZHUANG (庄杰), Ruonan MA (马若男), Zhen JIAO (焦浈). Comparative transcriptome analysis of atmospheric pressure cold plasma enhanced early seedling growth in Arabidopsis thaliana[J]. Plasma Science and Technology, 2021, 23(8): 85502-085502. DOI: 10.1088/2058-6272/ac0686

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Comparative transcriptome analysis of atmospheric pressure cold plasma enhanced early seedling growth in Arabidopsis thaliana

¹ Henan Key Laboratory of Ion-Beam Bioengineering, School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, People's Republic of China
² Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, People's Republic of China
³ Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, People's Republic of China

Funds: This work was supported by National Natural Science Foundation of China (Nos. 11605159 and 11405147), Chinese Postdoctoral Science Foundation (No. 2017M612412), the Foundation of Key Technology Research Project of Henan Province (No. 182102311115), Key Discipline Construction Project of Zhengzhou University (No. 32410257) and Youth Innovation Project of Key Discipline of Zhengzhou University (No. XKZDQN202002).

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Received Date: January 11, 2021
Revised Date: May 25, 2021
Accepted Date: May 27, 2021

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Abstract

Abstract

The stimulatory effects of atmospheric pressure cold plasma (APCP) on plant growth have attracted much attention due to its great potential as a new approach to increase crop growth and production. However, the transcriptome changes of plants induced by APCP treatment are unknown. Herein, the comparative transcriptome analysis was performed to identify the transcriptional response of Arabidopsis thaliana seedlings to APCP. Results showed that APCP exhibited a dual effect (stimulation or inhibition) on Arabidopsis seedling growth dependent on the treatment time and the maximum stimulatory effects were achieved by 1 min APCP treatment. The metabolic analysis of amino acid, glutathione (GSH) and phytohormone demonstrated that 1 min APCP treatment decreased most amino acids concentrations in Arabidopsis seedling, while the accumulations of GSH, gibberellins and cytokinin were significantly increased. The RNA-Seq analysis showed that a total of 218 differentially expressed genes (DEGs) were identified in 1 min APCP-treated seedlings versus the control, including 20 up-regulated and 198 down-regulated genes. The DEGs were enriched in pathways related to GSH metabolism, mitogen-activated protein kinase (MAPK) signaling transduction and plant resistance against pathogens. Moreover, most of the DEGs were defense, stimuli or stress-responsive genes and encoded proteins with oxidoreductase activity. Expression determination of six randomly selected DEGs by quantitative real-time PCR demonstrated similar pattern with the RNA-Seq data. These results indicated that the moderate APCP treatment may regulate the expression of stimuli/stress-responsive genes involved in GSH, phytohormone/amino metabolism and plant defense against pathogens via MAPK signal transduction pathway, accordingly enhance Arabidopsis seedling growth. This study provides a theoretical basis for the application of APCP in agriculture.

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References

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Comparative transcriptome analysis of atmospheric pressure cold plasma enhanced early seedling growth in Arabidopsis thaliana