Effect of plasma treatment on the seed germination and seedling growth of radish (<i>Raphanus sativus</i>)

Rajesh Prakash GURAGAIN; Hom Bahadur BANIYA; Santosh DHUNGANA; Ganesh Kuwar CHHETRI; Binita SEDHAI; Niroj BASNET; Aavash SHAKYA; Bishnu Prasad PANDEY; Suman Prakash PRADHAN; Ujjwal Man JOSHI; Deepak Prasad SUBEDI

doi:10.1088/2058-6272/ac3476

Plasma Science and Technology > 2022 > 24(1): 015502. > DOI: 10.1088/2058-6272/ac3476

Rajesh Prakash GURAGAIN, Hom Bahadur BANIYA, Santosh DHUNGANA, Ganesh Kuwar CHHETRI, Binita SEDHAI, Niroj BASNET, Aavash SHAKYA, Bishnu Prasad PANDEY, Suman Prakash PRADHAN, Ujjwal Man JOSHI, Deepak Prasad SUBEDI. Effect of plasma treatment on the seed germination and seedling growth of radish (Raphanus sativus)[J]. Plasma Science and Technology, 2022, 24(1): 015502. DOI: 10.1088/2058-6272/ac3476

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Effect of plasma treatment on the seed germination and seedling growth of radish (Raphanus sativus)

1.
Department of Physics, School of Sciences, Kathmandu University, Dhulikhel, Kavre 45210, Nepal
2.
Department of Physics, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu 44600, Nepal
3.
Department of Chemical Science and Engineering, Kathmandu University, Dhulikhel, Kavre 45210, Nepal
4.
Department of Environmental Science and Engineering, Kathmandu University, Dhulikhel, Kavre 45210, Nepal

More Information

Corresponding author:
Rajesh Prakash GURAGAIN, E-mail: rayessprakash@gmail.com

Hom Bahadur BANIYA, E-mail: hombaniya@gmail.com
Received Date: May 17, 2021
Revised Date: October 26, 2021
Accepted Date: October 27, 2021
Available Online: March 18, 2024
Published Date: November 21, 2021

Graphical Abstract

Abstract

Abstract

The effect on the germination and seedling growth of radish (Raphanus sativus) seeds were examined employing a dielectric barrier discharge (DBD) at atmospheric pressure and room temperature for various treatment time. DBD plasma using argon gas of flow rate 2 l m^-1 was employed in this study. Radish seeds were treated with DBD plasma for 1–5 min, respectively. Germination characteristics, seedling growth parameters, the contact angle of the seed coat, water uptake capacity, mass loss, the temperature of the seeds, chlorophyll, and carotenoid contents of the seedlings were measured before and after the DBD plasma treatments. Plasma treatment of radish seeds significantly increased germination-related characters, including germination percentage, fresh and dry weight, vigor index, and total carotenoids contents. However, the cumulative production rate was found to be decreased. Results from the experiment indicate an acceleration in the water uptake of the radish seeds and make the seed surface hydrophilic by plasma treatment. Scanning electron microscopy analysis showed that etching effects on the seed coat occurred after the argon plasma treatments, which affected the wettability of the radish seed. The experimental findings showed that seeds being treated by DBD plasma for 2 and 3 min had a positive effect on the germination and seedling growth of radish.
- dielectric barrier discharge (DBD),
- electron temperature,
- electron density,
- germination,
- growth parameters,
- radish seeds

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Acknowledgments

The authors are very thankful to Prof Dr Eun Ha Choi, and Dr Bhagirath Ghimire (Kwangwoon University, Korea), Milan Simek (Institute of Plasma Physics, The Czech Academy of Sciences), and Dr Johannes Gruenwald (Gruenwald Laboratories) for their valuable help and support. The corresponding author would also like to thank Nepal Academy of Science and Technology (NAST), Khumaltar, Lalitpur, Nepal for providing financial and technical support. The authors are also grateful to all the researchers of the Department of Physics, Kathmandu University who provided valuable suggestions for the completion of this work.

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